WebUse of CCSB Human Lentiviral ORF Library for pooled overexpression screening See reference paper from Dunn et al. and their supplemental protocol information (pdf downloads below) how to use this collection as pooled ORF library for over expression screening. Related Links Human ORFeome v8.1 Collection Downloads - Will open in new browser … Webin the CCSB-Broad Lentiviral Expression library, infection efficiency was expressed as the ratio of raw luminescence for cells selected with blasticidin divided by the raw luminescence for unselected cells (DMSO-treated). ORFs with an infection efficiency of less than 0.65 were considered failed infections
Sigma Mission TRC1 lentiviral shRNA Library – Duke Functional …
WebThe library is premised on the CCSB – Broad lentiviral ORF collection and was tested via V5 epitope tag expression. ~90% of ORF lentivirus induced expression greater than … WebThe CCSB-Broad Lentiviral Expression Library for human open reading frames. Choose from over 15,00 sequence-confirmed CMV-driven human ORFs with a C-terminal V5 tag … balada kpop rj
Lenti-cDNA Library Lenti-shRNA Core Facility - UNC …
WebYou can cherry-pick from our glycerol stock format sgRNA, shRNA, and mammalian over-expression reagents for human and mouse reagents to create the ideal clone collection for your particular needs. Here's how: Load gene identifiers for your desired reagents NCBI Gene ID or Gene Symbol Library reagent catalog numbers Select the reagent type WebEnter your username. Enter your password. Remember me WebControl or a CMV promoter-driven, lentiviral encoded vector to ectopically express RPL15. Lentiviral expression constructs for RPL15 (Accession: BC071672) were obtained from the CCSB-Broad Lentiviral Expression Library. Growth protocol arg dance